Anti-CKAP4 Antibody Picoband®

SKU:BHA21001086
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-Ckap4 antibody (Rabbit polyclonal, Rabbit IgG). Validated for Western blot. Reactivity: Mouse. Recognizes cytoskeleton-associated protein 4. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target Ckap4
Host Rabbit
Reactivity Mouse
Isotype Rabbit IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
A02154-1 100 ug/vial
Available Options

Select the variant that best fits your experiment.

  • Options:
    • 100 ug/vial / Carrier Free, Unconjugated
      Form: Lyophilized
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
      Applications: WB
      Application details: Western blot, 0.1-0.5μg/ml, Mouse
      Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
    • 100 ug/vial / APC, Biotin, Cy3, FITC, Fluoro488, Fluoro550, Fluoro594, Fluoro647, PE
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Applications: WB,IHC,ELISA; Flow Cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cellsContents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 100 ug/vial / HRP
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: varies by selected option; see option details under Options.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Ckap4
Alternative names Cytoskeleton-associated protein 4;63-kDa cytoskeleton-linking membrane protein;Climp-63;p63;Ckap4;
UniProt # Q8BMK4
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Mouse
Applications
  • Western Blot
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of mouse CKAP4, different from the related human sequence by one amino acid.
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Endoplasmic reticulum membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Translocates to the perinuclear region upon APF- stimulation. .
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) A02154-1
Main SKU BHA21001086
Rabbit Polyclonal · Picoband®

Product Overview

This rabbit polyclonal antibody targets cytoskeleton-associated protein 4 (gene Ckap4) in mouse samples and is validated for Western blot.

In mouse, cytoskeleton-associated protein 4 (Ckap4, also called CLIMP-63) is a membrane protein of the endoplasmic reticulum. It anchors ER sheets to microtubules and helps set the spacing of ER sheet membranes, and it is also studied as a cell-surface receptor.

Target Cytoskeleton-associated protein 4 (gene Ckap4; UniProt Q8BMK4, mouse)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Mouse
Purification Immunogen affinity purified
Form Lyophilized
Formulation Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers
Reconstitution 500 µg/mL after adding 0.2 mL distilled water
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 0.1–0.5 µg/mL (Mouse)

Immunogen

Synthetic peptide from the N-terminal region of mouse CKAP4. It differs from the human sequence at one residue.

Reactivity Notes

The supplier lists reactivity with mouse. UniProt places cytoskeleton-associated protein 4 in the endoplasmic reticulum membrane, cell membrane and cytoplasm. Tissue expression noted by UniProt (mouse): Expressed in cardiomyocytes (at protein level).

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 0.1–0.5 µg/mL (Mouse). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Mouse. Other species are not listed by the supplier.
Q.Has this antibody been tested on canine samples?
A.Reactivity with canine samples has not been tested by the supplier. Aligning the immunogen sequence with the canine ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide from the N-terminal region of mouse CKAP4. It differs from the human sequence at one residue.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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