Anti-Clathrin heavy chain/CLTC Antibody Picoband®

Overview

Anti-Clathrin heavy chain/CLTC Antibody Picoband® is an antibody for CLTC detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: CLTC (tumor necrosis factor receptor superfamily, member 18); UniProt: Q00610
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 192 kDa, calculated 26000 MW
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-Clathrin heavy chain/CLTC Antibody Picoband® catalog # A03134-1.

Biological background

Biological context: Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Expression and localization notes: cellular localization: Cell membrane. Single-pass type I membrane protein. Secreted., tissue context: Detected in thymus and in activated splenocytes..

Common research applications

• Western blotting (WB): Compare CLTC levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of CLTC in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify CLTC-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Clathrin heavy chain 1 is a protein that in humans is encoded by the CLTC gene. Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane. Single-pass type I membrane protein. Secreted.
• Tissue details: Detected in thymus and in activated splenocytes.
• Research category: Adaptive Immunity,Cancer,Cytokines,Growth Factors,Growth Factors/Hormones,Immunology,Innate Immunity,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.