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Cell Signaling
Product Overview
Biotinylated anti-Claudin-18 isoform 2 (CLDN18.2) IgG1 antibody is a purified recombinant antibody that recognizes human CLDN18.2 antigen. This antibody has been tested for specific binding to purified human CLDN18.2 protein.
| Clonality | Monoclonal |
|---|---|
| Host | Human |
| Antibody Type | IgG1 |
| Isotype | IgG1 |
| Conjugate | Biotin |
| Reactivity | Human |
| Purification | Protein A affinity |
| Purity | ≥90% |
| Molecular Weight | Total: 150 kDa; Light Chain: 24 kDa; Heavy Chain: 51 kDa |
| Formulation | 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol |
| UniProt | P56856 |
| Storage | At least 12 months at -80°C. |
Validated Applications
- Western Blot
Assay conditions: Functional validation: The antibody was validated by measuring anti-CLDN18.2 binding to CLDN18.2 antigen in ELISA. The CLDN18.2 protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) with DDM/CHS detergent (dodecyl-β-D-maltoside (DDM) 0.05%, cholesteryl hemisuccinate (CHS) 0.01%) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified biotinylated anti-CLDN18.2 antibody (BPS Bioscience #101565), serially diluted in Blocking Buffer 2 with DDM/CHS detergent, was added for 60 minutes at room temperature. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values. Flow cytometry validation: One million cells were stained with 1 µg of biotinylated anti-CLDN18.2 IgG for 30 minutes on ice, washed three times, stained with Streptavidin PE Conjugate (ThermoFisher #12-4317-87) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CLDN18.2 CHO cells (green) were compared to parental CHO-K1 cells (blue). Y-axis is the cell count. X-axis is PE intensity.
Immunogen / Antigen Information
Immunogen information not provided. Please refer to the product datasheet for detailed antigen information.
Cross-Reactivity & Specificity
Cross-reactivity data: Species reactivity as listed. No additional cross-reactivity data is available from the manufacturer.
Safety & Handling
Avoid freeze/thaw cycles.
For Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle with standard laboratory precautions (gloves, safety glasses, lab coat). Avoid freeze-thaw cycles. Consult SDS prior to use.
Related Products
- 78361
- 78527
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This antibody has been validated for: Western Blot. This product carries a Biotin label for direct detection. Optimal dilutions and concentrations should be empirically determined for each specific application and sample type. Refer to the product datasheet for suggested starting conditions.
This antibody should be stored at the indicated temperature to maintain stability. Store at At least 12 months at -80°C.. Avoid repeated freeze-thaw cycles, which can reduce antibody performance and increase aggregation. Centrifuge briefly after thawing and keep on ice during use.
Please refer to the BPS Bioscience datasheet for detailed immunogen information.
Cross-reactivity data is limited; refer to the product datasheet for available information.
BPS Bioscience offers multiple antibody formats for key checkpoint and immunotherapy targets. This product carries a Biotin label for direct detection. Contact BioHippo (support@biohippo.com) or click "Talk to a Scientist" to inquire about additional conjugate formats, bulk quantities, or custom formulations.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
