| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Claudin-2;SP82;CLDN2;PSEC0059, SP82, UNQ705/PRO1356; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Claudin 2 recombinant protein (Position: A38-V230). Human Claudin 2 shares 92% amino acid (aa) sequence identity with mouse Claudin 2. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CLDN2 (Claudin-2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Claudin 2/CLDN2 Antibody catalog # RP1042. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human Claudin 2 recombinant protein (Position: A38-V230). Human Claudin 2 shares 92% amino acid (aa) sequence identity with mouse Claudin 2. (reported region: A38-V230).
- Molecular weight context: reported MW: 25 kDa; calculated MW: 24549 MW
- Reactivity: Human,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Claudin-2; Claudin-2. Claudin-2 is a protein that in humans is encoded by the CLDN2 gene. By genomic sequence analysis, this gene is mapped to chromosome Xq22.3-q23. This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. By expression in a human intestinal epithelial cell line, it was determined that the intestine-specific homeodomain proteins CDX1 and CDX2 activated a reporter plasmid driven by the CLDN2 promoter. Functional note: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. . Reported localization: Cell junction, tight junction . Cell membrane ; Multi-pass membrane protein .
Research relevance and current trends
- Cell Adhesion: Researchers commonly examine how CLDN2 (Claudin-2) relates to this theme using model systems and orthogonal readouts.
- Cytoskeleton/ECM: Researchers commonly examine how CLDN2 (Claudin-2) relates to this theme using model systems and orthogonal readouts.
- Signal Transduction: Researchers commonly examine how CLDN2 (Claudin-2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CLDN2 (Claudin-2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CLDN2 (Claudin-2) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
