| Field | Specification |
|---|---|
| Target | |
| Alternative names | Claudin-5;Transmembrane protein deleted in VCFS;TMDVCF;CLDN5;AWAL, TMVCF; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Claudin 5, different from the related mouse sequence by three amino acids and from the related rat sequence by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets claudin-5 (gene CLDN5) in human and rat samples and is validated for Western blot. The predicted molecular weight is 23.1 kDa, and the supplier reports an observed band at about 23 kDa.
Claudin-5 (CLDN5) is the main claudin of endothelial tight junctions and is especially abundant in brain capillaries. By sealing the gaps between endothelial cells it is a key determinant of blood–brain barrier tightness, so it is a standard marker in studies of vascular permeability and neurovascular biology.
| Target | Claudin-5 (gene CLDN5; UniProt O00501, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 23.1 kDa |
| Observed MW | 23 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Rat, Human) |
|---|
Samples with a confirmed band (WB): rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the C-terminal region of human claudin 5. It differs from the mouse sequence at three residues.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places claudin-5 in the cell junction and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tian Jijing et al. (2019) Brain Infection by Hepatitis E Virus Probably via Damage of the Blood-Brain Barrier Due to Alterations of Tight Junction Proteins. Frontiers in Cellular and Infection Microbiology. 10.3389/fcimb.2019.00052
Fei Wang et al. (2020) HMGB1 promoted P-glycoprotein at the blood-brain barrier in MCAO rats via TLR4/NF-κB signaling pathway. European Journal of Pharmacology. 10.1016/j.ejphar.2020.173189
Bian-Sheng Ji et al. (2013) In vitro and in vivo study of dolichyl phosphate on the efflux activity of P-glycoprotein at the blood–brain barrier. International Journal of Developmental Neuroscience. 10.1016/j.ijdevneu.2013.10.005
Jian Wang et al. (2016) Effects of colistin on amino acid neurotransmitters and blood-brain barrier in the mouse brain. Neurotoxicology and Teratology. 10.1016/j.ntt.2016.03.004
Cen Juan et al. (2013) Alteration in P-glycoprotein at the blood–brain barrier in the early period of MCAO in rats. Journal of Pharmacy and Pharmacology. 10.1111/jphp.12033
