| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C-type lectin domain family 9 member A; CLEC9A; UNQ9341/PRO34046 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human CLEC9A, which shares 47.5% and 55% amino acid (aa) sequence identity with mouse and rat CLEC9A, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CLEC9A in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CLEC9A Antibody Picoband® catalog # A04577. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of human CLEC9A, which shares 47.5% and 55% amino acid (aa) sequence identity with mouse and rat CLEC9A, respectively.
- Molecular weight context: reported MW: 70 kDa; calculated MW: nan
- Reactivity: Human
- Applications: Flow Cytometry, IHC, ICC
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
C-type lectin domain containing 9A. C-type lectin domain family 9 member A is a protein that in humans is encoded by the CLEC9A gene. CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells. By genomic sequence analysis, this gene is mapped to chromosome 12p13.31, centromeric to CLEC12B (617573) and telomeric to CLEC1A (606782). Functional note: Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner. Reported localization: Membrane. Expression/tissue context: In peripheral blood highly restricted on the surface of BDCA31+ dendritic cells and on a small subset of CD14+ and CD16- monocytes.
Research relevance and current trends
- Hematopoietic Progenitors: Researchers commonly examine how CLEC9A relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how CLEC9A relates to this theme using model systems and orthogonal readouts.
- Innate Immunity: Researchers commonly examine how CLEC9A relates to this theme using model systems and orthogonal readouts.
Common research applications
- IHC/IHC-F: assess spatial distribution of CLEC9A across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
