| Field | Specification |
|---|---|
| Target | |
| Alternative names | Collagen alpha-1 (III) chain;COL3A1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen III, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects collagen alpha-1(III) chain (gene COL3A1) in human samples and is validated for Western blot. The predicted molecular weight is 138.6 kDa, and the supplier reports an observed band at about 39 kDa.
Collagen α1(III) chain (COL3A1) forms type III collagen, a fibrillar collagen that occurs with type I collagen in skin, blood vessels and hollow organs. It supports tissue elasticity and strength, and COL3A1 mutations cause vascular Ehlers-Danlos syndrome; it is also studied in wound healing and fibrosis.
| Target | Collagen alpha-1(III) chain (gene COL3A1; UniProt P02461, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 138.6 kDa |
| Observed MW | 39 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): MCF-4 cells, COLO320 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the C-terminal region of human collagen III. It differs from the mouse sequence at four residues and from the rat sequence at five residues.
Reactivity Notes
The supplier lists reactivity with human. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tong Wu et al. (2026) Ovarian Matrisome Dynamics and αvβ3-Mediated Regulation in Early Follicular Development. Advanced Science. 10.1002/advs.202507314
Tong Wu et al. (2022) Construction of Artificial Ovaries with Decellularized Porcine Scaffold and Its Elicited Immune Response after Xenotransplantation in Mice. Journal of Functional Biomaterials. 10.3390/jfb13040165
Wang Qing et al. (2018) YKL-40 is highly expressed in the epicardial adipose tissue of patients with atrial fibrillation and associated with atrial fibrosis. Journal of Translational Medicine. 10.1186/s12967-018-1598-0
