| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Collagen alpha-1 (IV) chain; Arresten; COL4A1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of COL4A1 (Collagen alpha-1(IV) chain) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Collagen IV/COL4A1 Antibody Picoband® catalog # PB9099. Tested in IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV. (reported region: G1445-T1669).
- Molecular weight context: reported MW: 250 kDa; calculated MW: 138564 MW
- Reactivity: Human
- Applications: IF, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Collagen alpha-1(IV) chain; collagen, type IV, alpha 1. COL4A1, also known as ICH or Collagen alpha-1 (IV), is a protein that in humans is encoded by the COL4A1 gene. It is mapped to 13q34. This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. COL4A1 binds to alpha-1/beta-1 integrin and inhibits migration, proliferation, and tube formation by endothelial cells. It is also a potential therapeutic candidate for targeting tumor angiogenesis. Functional note: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin. Reported localization: Basement membrane. Expression/tissue context: Highly expressed in placenta.
Research relevance and current trends
- Collagen: Researchers commonly examine how COL4A1 (Collagen alpha-1(IV) chain) relates to this theme using model systems and orthogonal readouts.
- Cytoskeleton/ECM: Researchers commonly examine how COL4A1 (Collagen alpha-1(IV) chain) relates to this theme using model systems and orthogonal readouts.
- ECM Proteins: Researchers commonly examine how COL4A1 (Collagen alpha-1(IV) chain) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative COL4A1 (Collagen alpha-1(IV) chain) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of COL4A1 (Collagen alpha-1(IV) chain) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the type IV collagen family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
