| Field | Specification |
|---|---|
| Target | |
| Alternative names | Collagen alpha-1 (IV) chain; Arresten; COL4A1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 3G3 is a mouse monoclonal antibody that recognizes collagen alpha-1(IV) chain (gene COL4A1) in human samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 160.6 kDa, and the supplier reports an observed band at about 220 kDa.
Collagen α1(IV) chain (COL4A1) is a component of type IV collagen, the network-forming collagen that builds basement membranes together with laminins, nidogen and proteoglycans. Its C-terminal fragment arresten has anti-angiogenic activity, and COL4A1 is studied in vascular biology and cerebral small vessel disease.
| Target | Collagen alpha-1(IV) chain (gene COL4A1; UniProt P02462, human) |
|---|---|
| Clone | 3G3 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 160.6 kDa |
| Observed MW | 220 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
Samples with a confirmed band (WB): human HEK293T cells, human HeLa cells, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant human Collagen IV fragment (Gly1445–Thr1669), expressed in E. coli. Sequence identity with the mouse ortholog: 97%.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Highly expressed in placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jiangang Liu et al. (2018) Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats. Molecular Medicine Reports. 10.3892/mmr.2018.8536
