| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Collagen alpha-1 (III) chain;Col3a1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Human collagen type III. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Collagen, Type III Col3a1 Antibody (Monoclonal, FH-7A) is an antibody targeting COL3A1. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: FH-7A; isotype: Mouse IgG1; reactivity: Human,Rat; observed MW: 49 kDa; calculated MW: 138936 MW.
Boster Bio Anti-Collagen, Type III Col3a1 Antibody (Monoclonal, FH-7A) catalog # MA1029. Tested in IHC, WB applications. This antibody reacts with Human, Rat.
Key elements and design rationale
- Target: COL3A1 — Collagen alpha-1(III) chain
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: FH-7A; Isotype: Mouse IgG1
- Species reactivity: Human,Rat
- Molecular weight guidance: Observed: 49 kDa; Calculated: 138936 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12 (By similarity). .
Scientific background (datasheet): Collagen type III, also known as COL3A1, is assigned to chromosome 2. Ultrastructural analysis of tissues from mutant mice revealed that type III collagen is essential for normal collagen I fibrillogenesis in the cardiovascular system and other organs. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development.
Cellular localization (datasheet): Secreted, extracellular space, extracellular matrix .
Tissue details (datasheet): Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue. .
Sequence similarities (datasheet): Belongs to the fibrillar collagen family.
Research relevance and current trends
- Commonly studied in contexts related to Collagen,Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Mesenchymal Stem Cells,Signal Transduction,Stem Cells.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
