Anti-Collagen, Type III Col3a1 Antibody (Monoclonal, FH-7A)

SKU:BHA21006513
—
Suppliers
Boster Bio
Boster Bio
Details Products
Overview
Click light‑blue chips for details
Anti-COL3A1 antibody (Mouse monoclonal, clone FH-7A, Mouse IgG1). Validated for Western blot and immunohistochemistry. Reactivity: Human, Rat. Recognizes collagen alpha-1(III) chain. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target COL3A1
Clone number FH-7A
Host Mouse
Reactivity Human,Rat
Isotype Mouse IgG1
Application(s) WB, IHC
Options selector
Catalog no. Size Conjugation
MA1029 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial — Unconjugated
      Contents: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
      Form: Lyophilized
      Applications: IHC,WB
      Application details: Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, rat, -
      Western blot, 0.25-0.5μg/ml, Human, rat
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target COL3A1
Alternative names Collagen alpha-1 (III) chain;Col3a1;
Gene ID Human(1281), Mouse(12825), Rat(84032)
UniProt # P13941
Host Mouse
Clone FH-7A
Clonality
  • Monoclonal
Isotype
  • Mouse IgG1
Reactivity
  • Human
  • Rat
Applications
  • Western Blot
  • Immunohistochemistry
Immunogen Human collagen type III.
Purification Ascites
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Cellular localization Secreted, extracellular space, extracellular matrix .
Concentration Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) MA1029
Main SKU BHA21006513
Mouse Monoclonal

Product Overview

Clone FH-7A is a mouse monoclonal antibody that recognizes collagen alpha-1(III) chain (gene Col3a1) in human and rat samples and is validated for Western blot and IHC.

Collagen α1(III) chain (COL3A1) forms type III collagen, a fibrillar collagen that occurs with type I collagen in skin, blood vessels and hollow organs. It supports tissue elasticity and strength, and COL3A1 mutations cause vascular Ehlers-Danlos syndrome; it is also studied in wound healing and fibrosis.

Target Collagen alpha-1(III) chain (gene Col3a1; UniProt P13941, rat)
Clone FH-7A
Host / Clonality / Isotype Mouse / Monoclonal / Mouse IgG1
Reactivity Human, Rat
Purification Ascites
Form Lyophilized
Formulation 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers
Reconstitution 100 µg/mL after adding 1 mL PBS
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 0.25–0.5 µg/mL (Human, Rat)
Immunohistochemistry Not specified by the supplier

Immunogen

Human collagen type III.

Reactivity Notes

The supplier lists reactivity with human and rat.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 0.25–0.5 µg/mL (Human, Rat); Immunohistochemistry (dilution not specified). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Rat. Other species are not listed by the supplier.
Q.Has this antibody been tested on canine samples?
A.Reactivity with canine samples has not been tested by the supplier. Aligning the immunogen sequence with the canine ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.How is this Collagen III monoclonal antibody supplied?
A.According to the supplier: it is supplied lyophilized as unpurified mouse ascites. Each vial contains 100 µg of target-specific antibody plus protective carrier protein at a 1:20 ratio.
Q.Is a carrier-free (BSA-free) version available, for example for conjugation?
A.Yes. A carrier-free formulation without BSA, stabilized with trehalose and/or glycerol, can be prepared on request and is compatible with common conjugation chemistries. Please note this when ordering or contact info@biohippo.com. For storage at −20 °C, keep a cryoprotectant such as glycerol in the buffer rather than PBS alone.
Q.What immunogen was used to raise this antibody?
A.Human collagen type III.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Get a Quote

Please use this form for bulk quantity requests or customized products.

Contact Information

Product Information

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today