| Field | Specification |
|---|---|
| Target | |
| Alternative names | Src substrate cortactin; Amplaxin; Oncogene EMS1; CTTN; EMS1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Cortactin recombinant protein (Position: M1-Q105). |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes src substrate cortactin (gene CTTN) in human, mouse and rat samples and is validated for ELISA, flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 61.6 kDa, and the supplier reports an observed band at about 85 kDa.
Cortactin (CTTN) is an actin-binding protein that activates the Arp2/3 complex and stabilizes branched actin networks. It is enriched in lamellipodia, invadopodia and sites of endocytosis, and its phosphorylation by SRC and other kinases regulates cell migration. It is studied in cell motility, cancer invasion, neuronal morphology and focal adhesion dynamics.
| Target | Src substrate cortactin (gene CTTN; UniProt Q14247, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 61.6 kDa |
| Observed MW | 85 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| IHC (frozen sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A431 cells, human U-87MG cells, human A549 cells, human PC-3 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human oesophagus squama cancer tissue, human cholangiocarcinoma tissue, human gastric cancer tissue, human glioma tissue, human ovary cancer tissue, human sarcoma tissue, human appendicitis tissue, human liver cancer tissue, human lung cancer tissue, human placenta tissue, human rectal cancer tissue, mouse intestine tissue, mouse spleen tissue, rat brain tissue, rat intestine tissue, rat spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (IHC): human placenta tissue.
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Recombinant human Cortactin fragment (Met1–Gln105), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places src substrate cortactin in the cytoplasm, cell projection and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tongyao Yu et al. (2025) Rotating magnetic field downregulating type XI collagen to suppress triple-negative breast cancer metastasis by inactivating the ITGB1/FAK/YAP signaling pathway. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.149677
Zhang Shilong et al. (2024) Systematic simulation of tumor cell invasion and migration in response to time-varying rotating magnetic field. Biomechanics and Modeling in Mechanobiology. 10.1007/s10237-024-01858-y
