| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytochrome c oxidase subunit 8A, mitochondrial; Cytochrome c oxidase polypeptide VIII-liver; Cytochrome c oxidase subunit 8-2; Cox8a; Cox8; Cox8l |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse COX8A, which shares 100% and 66.7% amino acid (aa) sequence identity with human and rat COX8A, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes cytochrome c oxidase subunit 8A, mitochondrial (gene Cox8a) in mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 7.6 kDa, and the supplier reports an observed band at about 8–10 kDa.
In mouse, Cox8a is the ubiquitously expressed isoform of cytochrome c oxidase subunit 8. It is a small nuclear-encoded component of complex IV, the enzyme that transfers electrons to oxygen at the end of the respiratory chain, and its mitochondrial targeting sequence is widely used to direct reporters to mitochondria.
| Target | Cytochrome c oxidase subunit 8A, mitochondrial (gene Cox8a; UniProt Q64445, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 7.6 kDa |
| Observed MW | 8–10 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
Samples with a confirmed band (WB): rat brain tissue, rat stomach tissue, rat kidney tissue, mouse brain tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): mouse spleen tissue.
Immunogen
Synthetic peptide from the C-terminal region of mouse COX8A. Sequence identity with the human and rat orthologs: 100% and 66.7%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places cytochrome c oxidase subunit 8A, mitochondrial in the mitochondrion inner membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
