| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8; CPAMD8; KIAA1283 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CPAMD8 recombinant protein (Position: R58-D234). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CPAMD8 in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CPAMD8 Antibody Picoband® catalog # A12898. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human CPAMD8 recombinant protein (Position: R58-D234). (reported region: R58-D234).
- Molecular weight context: reported MW: 207 kDa; calculated MW: nan
- Reactivity: Human,Monkey
- Applications: ELISA, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
C3 and PZP like, alpha-2-macroglobulin domain containing 8. CPAMD8 belongs to the complement component-3 (C3; 120700)/alpha-2-macroglobulin (A2M; 103950) family of proteins, which are involved in innate immunity and damage control. The encoded protein is membrane-associated and proteolytically processed to generate two chains. Mutations in this gene cause a form of anterior segment dysgenesis, a developmental disorder of the eye. By genomic sequence analysis, the CPAMD8 gene is mapped to chromosome 19p13.3-p13.2. Functional note: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Reported localization: Secreted. Cell membrane. Peripheral membrane protein. Extracellular side. Expression/tissue context: Highly expressed in the kidney, brain and testis and to a lower extent in heart, liver and small intestine. Expressed in the lens, cornea and retina. Strongly expressed in the distal tips of the retinal neuroepithelium that form the iris and ciliary body.
Research relevance and current trends
- Complement: Researchers commonly examine how CPAMD8 relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how CPAMD8 relates to this theme using model systems and orthogonal readouts.
- Innate Immunity: Researchers commonly examine how CPAMD8 relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CPAMD8 levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CPAMD8 across tissue regions and cell types using matched controls.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
