| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein phosphatase 1 regulatory subunit 14A;17 kDa PKC-potentiated inhibitory protein of PP1;Protein kinase C-potentiated inhibitor protein of 17 kDa;CPI-17;PPP1R14A;CPI17, PPP1INL; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CPI17 alpha recombinant protein (Position: L30-Q126). Human CPI17 alpha shares 83.5% and 84.5% amino acid (aa) sequence identity with mouse and rat CPI17 alpha, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CPI17 alpha/PPP1R14A Antibody Picoband® catalog # A05752-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human CPI17 alpha recombinant protein (Position: L30-Q126). Human CPI17 alpha shares 83.5% and 84.5% amino acid (aa) sequence identity with mouse and rat CPI17 alpha, respectively. (reported region: L30-Q126).
- Molecular weight context: reported MW: 20 kDa; calculated MW: 16693 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Protein phosphatase 1 regulatory subunit 14A; Protein phosphatase 1 regulatory subunit 14A. Protein phosphatase 1 regulatory subunit 14A, also known as CPI-17, is a protein that in humans is encoded by the PPP1R14A gene. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. Functional note: Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. Reported localization: Cytoplasm . Expression/tissue context: Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta. .
Research relevance and current trends
- Protein Phosphorylation: Researchers commonly examine how PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) relates to this theme using model systems and orthogonal readouts.
- Ser/Thr Kinases: Researchers commonly examine how PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) relates to this theme using model systems and orthogonal readouts.
- Signal Transduction: Researchers commonly examine how PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
