| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | DNA replication licensing factor MCM5; CDC46 homolog; P1-CDC46; MCM5; CDC46 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CPO recombinant protein (Position: D34-V280). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CPO Antibody Picoband® is an antibody for CPO detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: CPO (minichromosome maintenance complex component 5); UniProt: Q8IVL8
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 57 kDa, calculated 28461 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-CPO Antibody Picoband® catalog # A03649-1.
Biological background
Biological context: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Interacts with MCMBP.
Expression and localization notes: cellular localization: Nucleus. Cytosol., tissue context: Ubiquitous..
Common research applications
- Western blotting (WB): Compare CPO levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of CPO in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify CPO-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: This gene is a member of the metallocarboxypeptidase gene family.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus. Cytosol.
- Tissue details: Ubiquitous.
- Research category: ER Proteins,Golgi Proteins,Protein Trafficking,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
