| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein crumbs homolog 1; CRB1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CRB1, which shares 87.9% and 84.8% amino acid (aa) sequence identity with mouse and rat CRB1, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CRB1 Antibody Picoband® is an antibody for CRB1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: CRB1 (crumbs 1, cell polarity complex component); UniProt: P82279
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 154 kDa, calculated 75679 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-CRB1 Antibody Picoband® catalog # A01499-1.
Biological background
Biological context: Plays a role in photoreceptor morphogenesis in the retina. May maintain cell polarization and adhesion.
Expression and localization notes: cellular localization: Apical cell membrane. Single-pass type I membrane protein. Secreted., tissue context: Preferential expression in retina, also expressed in brain, testis, fetal brain and fetal eye..
Common research applications
- Western blotting (WB): Compare CRB1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of CRB1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify CRB1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Crumbs homolog 1 is a protein that in humans is encoded by the CRB1 gene. This gene encodes a protein which is similar to the Drosophila crumbs protein and localizes to the inner segment of mammalian photoreceptors. In Drosophila crumbs localizes to the stalk of the fly photoreceptor and may be a component of the molecular scaffold that controls proper development of polarity in the eye. Mutations in this gene are associated with a severe form of retinitis pigmentosa, RP12, and with Leber congenital amaurosis. Alternate splicing results in multiple transcript variants, some protein coding and some non-protein coding.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Apical cell membrane. Single-pass type I membrane protein. Secreted.
- Tissue details: Preferential expression in retina, also expressed in brain, testis, fetal brain and fetal eye.
- Research category: Cell Biology,Cell Cycle,Epigenetics and Nuclear Signaling,Kinases/Phosphatases,Mediator Complex,Transcription
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
