| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclic AMP-responsive element-binding protein 1;CREB-1;cAMP-responsive element-binding protein 1;CREB1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CREB recombinant protein (Position: M1-T177). Human CREB shares 99% and 98% amino acid (aa) sequences identity with mouse and rat CREB, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects cyclic AMP-responsive element-binding protein 1 (gene CREB1) in human, mouse and rat samples and is validated for flow cytometry and Western blot. The predicted molecular weight is 36.7 kDa, and the supplier reports an observed band at about 43 kDa.
CREB (CREB1) is a transcription factor that binds cAMP response elements (CRE) in gene promoters. Phosphorylation at Ser133 by PKA and other kinases recruits the coactivators CBP and p300 to switch on target genes, and CREB is widely studied in neuronal plasticity, memory, metabolism and cell survival.
| Target | Cyclic AMP-responsive element-binding protein 1 (gene CREB1; UniProt P16220, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 36.7 kDa |
| Observed MW | 43 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human 293T cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Recombinant human CREB fragment (Met1–Thr177), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 99% and 98%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cyclic AMP-responsive element-binding protein 1 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ünal Gülin Özdamar et al. (2023) The beneficial effects of vortioxetine on BDNF, CREB, S100B, β amyloid, and glutamate NR2b receptors in chronic unpredictable mild stress model of depression. Psychopharmacology. 10.1007/s00213-023-06445-0
Ma Yongkang et al. (2026) The influence of HINT1 protein in nucleus accumbens on aggressive behavior and anxiety-like behavior. Psychopharmacology. 10.1007/s00213-026-07102-y
Jun Shen et al. (2018) Resveratrol prevents cognitive deficits induced by chronic unpredictable mild stress: Sirt1/miR-134 signalling pathway regulates CREB/BDNF expression in hippocampus in vivo and in vitro. Behavioural Brain Research. 10.1016/j.bbr.2018.04.050
Ping Yang et al. (2018) Buyang huanwu decoction combined with BMSCs transplantation promotes recovery after spinal cord injury by rescuing axotomized red nucleus neurons. Journal of Ethnopharmacology. 10.1016/j.jep.2018.09.028
Ming-Lian Luo et al. (2026) Single administration of vitamin C produces rapid antidepressant-like effects in female mice: A possible role of dopamine D2 receptor signalling. Brain Research. 10.1016/j.brainres.2026.150181
Y. Tan et al. (2005) Traditional Chinese medicine Bao Gan Ning increase phosphorylation of CREB in liver fibrosis in vivo and in vitro. Journal of Ethnopharmacology. 10.1016/j.jep.2005.09.040
Chunqiang Wang et al. (2019) Nerve growth factor regulates the proliferation of cashmere goat outer root sheath cells through the activation of cAMP-binding protein. Small Ruminant Research. 10.1016/j.smallrumres.2019.06.005
