| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclic AMP-responsive element-binding protein 3-like protein 1;cAMP-responsive element-binding protein 3-like protein 1;Old astrocyte specifically-induced substance;OASIS;Processed cyclic AMP-responsive element-binding protein 3-like protein 1;CREB3L1;OASIS;PSEC0238; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CREB3L1 recombinant protein (Position: M1-M204). Human CREB3L1 shares 93.6% and 93.1% amino acid (aa) sequence identity with mouse and rat CREB3L1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets cyclic AMP-responsive element-binding protein 3-like protein 1 (gene CREB3L1) in human samples and is validated for Western blot. The predicted molecular weight is 57.0 kDa, and the supplier reports an observed band at about 57 kDa.
CREB3L1 (also called OASIS) is an ER-bound transcription factor of the CREB3 family. When released by regulated proteolysis it enters the nucleus and activates genes such as type I collagen, playing a key role in osteoblast function and bone formation, and it is studied in ER stress and secretory cells.
| Target | Cyclic AMP-responsive element-binding protein 3-like protein 1 (gene CREB3L1; UniProt Q96BA8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 57.0 kDa |
| Observed MW | 57 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human placenta tissue, HeLa cells, A549 cells, MM231 cells, COLO320 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human CREB3L1 fragment (Met1–Met204), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93.6% and 93.1%, respectively.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Expressed in several tissues, with highest levels in pancreas and prostate. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
