| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-crystallin A chain; Heat shock protein beta-4; HspB4; Heat shock protein family B member 4; CRYAA; CRYA1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CRYAA |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23C19 is a rabbit monoclonal antibody that recognizes alpha-crystallin A chain (gene CRYAA) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 19.9 kDa, and the supplier reports an observed band at about 17 kDa.
αA-crystallin (CRYAA) is a small heat shock protein and a major structural protein of the eye lens, where it helps maintain transparency and refractive index. It also acts as a chaperone that prevents protein aggregation under stress, and CRYAA mutations cause congenital cataract.
| Target | Alpha-crystallin A chain (gene CRYAA; UniProt P02489, human) |
|---|---|
| Clone | 23C19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 19.9 kDa |
| Observed MW | 17 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human CRYAA.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places alpha-crystallin A chain in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in the eye lens (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
