| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cold shock domain-containing protein E1; N-ras upstream gene protein; Protein UNR; CSDE1; D1S155E; KIAA0885; NRU; UNR |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CSDE1/NRU recombinant protein (Position: M1-Q775). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets cold shock domain-containing protein E1 (gene CSDE1) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 88.9 kDa, and the supplier reports an observed band at about 89 kDa.
CSDE1 (also called UNR) is a cytoplasmic RNA-binding protein with several cold shock domains. It helps couple translation with mRNA decay, for example of FOS mRNA, and is needed for efficient stress granule assembly. It also supports internal ribosome entry on some viral RNAs, such as human rhinovirus, so it is studied in post-transcriptional gene control and virology.
| Target | Cold shock domain-containing protein E1 (gene CSDE1; UniProt O75534, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 88.9 kDa |
| Observed MW | 89 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human MCF-7 cells, human U-87MG cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): THP-1 cells.
Immunogen
Recombinant human CSDE1/NRU fragment (Met1–Gln775), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places cold shock domain-containing protein E1 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
