| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Sodium- and chloride-dependent GABA transporter 1;GAT-1;Solute carrier family 6 member 1;SLC6A1;GABATR, GABT1, GAT1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CSDE1/NRU recombinant protein (Position: M1-Q775). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CSDE1/NRU Antibody Picoband® is an antibody for CSDE1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: CSDE1 (Sodium- and chloride-dependent GABA transporter 1); UniProt: O75534
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 89 kDa, calculated 67074 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-CSDE1/NRU Antibody Picoband® catalog # A05114-1.
Biological background
Biological context: Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
Expression and localization notes: cellular localization: Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons (By similarity). ., tissue context: Expressed in many tissues, highest levels in skeletal muscle..
Common research applications
- Western blotting (WB): Compare CSDE1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of CSDE1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify CSDE1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Cold shock domain-containing protein E1 is a protein that in humans is encoded by the CSDE1 gene. Originally described as the product of an active transcription unit located upstream of the N-RAS gene, CSDE1 (cold shock domain-containing protein E1) is a highly conserved RNA-binding protein containing five cold-shock domains. CSDE1 is mostly localized in the cytoplasm where it interacts with distinct protein complexes involved in the post-transcriptional regulation of numerous mRNAs in a dynamic and complex manner. CSDE1 plays a role in maintaining the undifferentiated state of human embryonic stem cells (hESCs), and its loss results in accelerated neural differentiation and neurogenesis, partially via post-transcriptional regulation of fatty acid binding protein 7 (FABP7) and vimentin (VIM) mRNAs. In human melanoma, increased CSDE1 protein expression has been shown to modulate the levels of pro-oncogenic factors such as vimentin or RAC1 and tumor suppressors such as PTEN, thus promoting tumor invasion and metastasis.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons (By similarity). .
- Tissue details: Expressed in many tissues, highest levels in skeletal muscle.
- Research category: Neuroscience,Neurotransmitter,Transporters
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
