| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tyrosine-protein kinase CSK;2.7.10.2;C-Src kinase;Protein-tyrosine kinase CYL;CSK; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CSK recombinant protein (Position: S2-G204). Human CSK shares 99% amino acid (aa) sequence identity with both mouse and rat CSK. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CSK (Tyrosine-protein kinase CSK) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Csk Antibody Picoband® catalog # PB9102. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human CSK recombinant protein (Position: S2-G204). Human CSK shares 99% amino acid (aa) sequence identity with both mouse and rat CSK. (reported region: S2-G204).
- Molecular weight context: reported MW: 51 kDa; calculated MW: 50704 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Tyrosine-protein kinase CSK; Tyrosine-protein kinase CSK. CSK also known as C-Src kinase, is a non-receptor protein tyrosine kinase protein that in humans is encoded by the CSK gene. It is mapped to 15q24.1. CSK suppresses activity of the Src family of protein kinases by phosphorylation of Src family members at a conserved C-terminal site in Src. Csk's control of the Src family activity is widely thought to be central to regulation of the immune response. CSK can downregulate tyrosine kinase activity of the SRC oncoprotein through tyrosine phosphorylation of the SRC carboxy terminus. Since cell transformation by SRC oncoproteins is caused by various mechanisms that interfere with this phosphorylation, the CSK gene might function as an antioncogene. Functional note: Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T- cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK. . Reported localization: Cytoplasm . Cell membrane . Mainly cytoplasmic, also present in lipid rafts. . Expression/tissue context: Expressed in lung and macrophages. .
Research relevance and current trends
- Protein Phosphorylation: Researchers commonly examine how CSK (Tyrosine-protein kinase CSK) relates to this theme using model systems and orthogonal readouts.
- Signal Transduction: Researchers commonly examine how CSK (Tyrosine-protein kinase CSK) relates to this theme using model systems and orthogonal readouts.
- Tyrosine Kinases: Researchers commonly examine how CSK (Tyrosine-protein kinase CSK) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CSK (Tyrosine-protein kinase CSK) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CSK (Tyrosine-protein kinase CSK) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
