| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein kinase CSK;2.7.10.2;C-Src kinase;Protein-tyrosine kinase CYL;CSK; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CSK recombinant protein (Position: S2-G204). Human CSK shares 99% amino acid (aa) sequence identity with both mouse and rat CSK. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets tyrosine-protein kinase CSK in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 50.7 kDa, and the supplier reports an observed band at about 51 kDa.
C-terminal Src kinase (CSK) is a non-receptor tyrosine kinase that switches off Src-family kinases such as SRC, LCK and FYN. It phosphorylates a tyrosine in their C-terminal tail, which folds the kinases into an inactive conformation. By limiting LCK and FYN, CSK sets the threshold for T-cell and B-cell receptor signaling and also affects cell growth and migration.
| Target | Tyrosine-protein kinase CSK (gene CSK; UniProt P41240, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 50.7 kDa |
| Observed MW | 51 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): rat testis tissue, rat thymus tissue, mouse liver tissue, human HeLa cells, human Jurkat cells, human A549 cells, human MCF-7 cells, mouse NIH/3T3 cells, mouse NEURO cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant human CSK fragment (Ser2–Gly204), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 99%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tyrosine-protein kinase CSK in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Expressed in lung and macrophages. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wei Wu et al. (2020) Multiple Signaling Roles of CD3ε and Its Application in CAR-T Cell Therapy. Cell. 10.1016/j.cell.2020.07.018
