| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-terminal-binding protein 2; CtBP2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CTBP2 recombinant protein (Position: H321-Q445). human CTBP2 shares 99.2% and 98.4% amino acid (aa) sequence identity with mouse and rat CTBP2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 7F3E1 is a mouse monoclonal antibody that recognizes C-terminal-binding protein 2 (gene CTBP2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 48.9 kDa, and the supplier reports an observed band at about 49 kDa.
CtBP2 is a transcriptional corepressor closely related to CtBP1 that binds diverse DNA-binding repressors and helps silence their target genes. An alternative isoform, RIBEYE, is a main structural protein of ribbon synapses in retinal photoreceptors and inner-ear hair cells, so CTBP2 antibodies are widely used as ribbon synapse markers.
| Target | C-terminal-binding protein 2 (gene CTBP2; UniProt P56545, human) |
|---|---|
| Clone | 7F3E1 |
| Host / Clonality / Isotype | Mouse / Monoclonal / IgG2a |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 48.9 kDa |
| Observed MW | 49 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human SiHa cells, human COLO 320 cells, human SW620 cells ysates, rat stomach tissue, rat brain tissue, mouse stomach tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human colorectal adenocarcinoma tissue, human liver cancer tissue, human placenta tissue, human thyroid cancer tissue, human laryngeal squamous cells carcinoma tissue, mouse colon tissue, rat colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): mouse testis tissue, mouse colon tissue.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Recombinant human CTBP2 fragment (His321–Gln445), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 99.2% and 98.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places C-terminal-binding protein 2 in the nucleus and synapse. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liqin Wang et al. (2024) Amitriptyline protects afferent synapses in the cochlea against excitotoxic trauma in vitro. FEBS Journal. 10.1111/febs.17233
