| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Connective tissue growth factor;CCN family member 2;Hypertrophic chondrocyte-specific protein 24;Insulin-like growth factor-binding protein 8;IBP-8;IGF-binding protein 8;IGFBP-8;CTGF;CCN2, HCS24, IGFBP8; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CTGF recombinant protein (Position: R58-A349). Human CTGF shares 95.5% and 95.9% amino acid (aa) sequence identity with mouse and rat CTGF, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CTGF (Connective tissue growth factor) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CTGF Antibody Picoband® catalog # PB9541. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human CTGF recombinant protein (Position: R58-A349). Human CTGF shares 95.5% and 95.9% amino acid (aa) sequence identity with mouse and rat CTGF, respectively. (reported region: R58-A349).
- Molecular weight context: reported MW: 38 kDa; calculated MW: 38091 MW
- Reactivity: Human,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Connective tissue growth factor; Connective tissue growth factor. CTGF, also known as CCN2 or connective tissue growth factor, is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins (see also CCN intercellular signaling protein). CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. And the encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. Functional note: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. . Reported localization: Secreted, extracellular space, extracellular matrix . Secreted . Expression/tissue context: Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested. .
Research relevance and current trends
- Angiogenesis: Researchers commonly examine how CTGF (Connective tissue growth factor) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how CTGF (Connective tissue growth factor) relates to this theme using model systems and orthogonal readouts.
- Growth Factors: Researchers commonly examine how CTGF (Connective tissue growth factor) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CTGF (Connective tissue growth factor) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CTGF (Connective tissue growth factor) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the CCN family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
