Anti-Cullin 1/CUL1 Antibody Picoband®

Overview

This antibody is intended for detection of CUL1 (Cullin-1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-Cullin 1/CUL1 Antibody Picoband® catalog # PB9542. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: E.coli-derived human Cullin 1 recombinant protein (Position: E457-D770). Human Cullin 1 shares 100% amino acid (aa) sequence identity with mouse Cullin 1. (reported region: E457-D770).
• Molecular weight context: reported MW: 90 kDa; calculated MW: 89679 MW
• Reactivity: Human,Mouse,Rat
• Applications: WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Cullin-1; Cullin-1. Cullin 1, also known as CUL1, is a human protein and gene from cullin family. This protein plays an important role in protein degradation and protein ubiquitination. This is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Human cullin 1, but not the other closely related cullins 2, 3, 4A, and 5, selectively interacts with human SKP1. Functional note: Core component of multiple cullin-RING-based SCF (SKP1- CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF (BTRC) and SCF (FBXW11) ubiquitination of CTNNB1 and participate in Wnt signaling. SCF (FBXW11) s ubiquitination of phosphorylated NFKBIA. SCF (BTRC) s ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF (SKP2) s ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF (SKP2) s ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF (FBXW7) s ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF (FBXW2) s ubiquitination of GCM1. SCF (FBXO32) s ubiquitination of MYOD1. SCF (FBXO7) s ubiquitination of BIRC2 and DLGAP5. SCF (FBXO33) s ubiquitination of YBX1. SCF (FBXO1) s ubiquitination of BCL6 and DTL but does not seem to ubiquitination of TP53. SCF (BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF (CCNF) s ubiquitination of CCP110. SCF (FBXL3) and SCF (FBXL21) ubiquitination of CRY1 and CRY2. SCF (FBXO9) ubiquitination of TTI1 and TELO2. SCF (FBXO10) s ubiquitination of BCL2. . Reported localization: Secreted, extracellular space, extracellular matrix . Secreted . Expression/tissue context: Expressed in lung fibroblasts. .

Research relevance and current trends

• Apoptosis: Researchers commonly examine how CUL1 (Cullin-1) relates to this theme using model systems and orthogonal readouts.
• Cancer: Researchers commonly examine how CUL1 (Cullin-1) relates to this theme using model systems and orthogonal readouts.
• Cell Cycle: Researchers commonly examine how CUL1 (Cullin-1) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative CUL1 (Cullin-1) levels across conditions; band patterns may reflect isoforms and processing.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins
• Family / similarity context: Belongs to the CCN family.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.