| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cadherin-2;CDw325;Neural cadherin;N-cadherin;CD325;CDH2;CDHN, NCAD; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Cullin4A Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-CUL4A antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AEIH-3; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-Cullin4A Monoclonal Antibody catalog # M01579. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CUL4A (Cadherin-2).
- Antibody format: Monoclonal; clone AEIH-3; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
CUL4A (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity). . Reported cellular localization context: Cell membrane; Single-pass type I membrane protein. Tissue expression notes (as provided): Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts. .
Research relevance and current trends
- Research context keywords from the source record include: Ubiquitin E3 Enzymes.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate CUL4A antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CUL4A expression by Western blot in cell or tissue lysates, Detect CUL4A in FFPE tissue sections by immunohistochemistry, Localize CUL4A by immunofluorescence/immunocytochemistry in cultured cells, Quantify CUL4A-positive cells by flow cytometry in single-cell suspensions, Enrich CUL4A by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 75 kDa; calculated MW: 99809 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 75 kDa
- Cellular localization (provided): Cell membrane; Single-pass type I membrane protein.
- Tissue details (provided): Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
