| Field | Specification |
|---|---|
| Target | |
| Alternative names | Coxsackievirus and adenovirus receptor; CAR; hCAR; CVB3-binding protein; Coxsackievirus B-adenovirus receptor; HCVADR; CXADR |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CXADR recombinant protein (Position: E35-V365). Human CXADR shares 91.2% and 91.5% amino acid (aa) sequence identity with mouse and rat CXADR, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects coxsackievirus and adenovirus receptor (gene CXADR) in human, monkey, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA.
CAR (CXADR) is a transmembrane protein of tight junctions and the receptor used by coxsackie B viruses and many adenoviruses to enter cells. It mediates homophilic cell adhesion, supports epithelial barrier integrity and, through binding JAML, helps leukocytes cross epithelia and activates γδ T cells. It is also needed for heart development and is studied in virology and gene therapy.
| Target | Coxsackievirus and adenovirus receptor (gene CXADR; UniProt P78310, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Monkey, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg /1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human CXADR fragment (Glu35–Val365), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 91.2% and 91.5%, respectively.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. Tissue expression noted by UniProt (human): Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaowei Zhang et al. (2013) Human Astrocytic Cells Support Persistent Coxsackievirus B3 Infection. Journal of Virology. 10.1128/JVI.02090-13
