| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C-X-C motif chemokine 16;Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein;SR-PSOX;Small-inducible cytokine B16;Transmembrane chemokine CXCL16;CXCL16;SCYB16, SRPSOX;UNQ2759/PRO6714; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E. coli-derived human CXCL16 recombinant protein (Position: N30-A196). Human CXCL16 shares 47% and 45.7% amino acid (aa) sequence identity with mouse and rat CXCL16, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CXCL16 (C-X-C motif chemokine 16) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CXCL16 Antibody Picoband® catalog # PB10054. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human CXCL16 recombinant protein (Position: N30-A196). Human CXCL16 shares 47% and 45.7% amino acid (aa) sequence identity with mouse and rat CXCL16, respectively. (reported region: N30-A196).
- Molecular weight context: reported MW: 35 kDa; calculated MW: 27579 MW
- Reactivity: Human
- Applications: ELISA, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
C-X-C motif chemokine 16; C-X-C motif chemokine 16. CXCL16, Chemokine (C-X-C motif) ligand 16, is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. By somatic cell hybrid analysis, the CXCL16 gene is mapped to 17p13, a locus separate from all other known chemokines. Chemotaxis assays found that CXCL16 induced a strong chemotactic response in activated CD8 T cells. In addition, CXCL16 induced calcium mobilization. Functional note: Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. . Reported localization: Cell membrane ; Single-pass type I membrane protein . Secreted. Also exists as a soluble form. Expression/tissue context: Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.
Research relevance and current trends
- Chemokines: Researchers commonly examine how CXCL16 (C-X-C motif chemokine 16) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how CXCL16 (C-X-C motif chemokine 16) relates to this theme using model systems and orthogonal readouts.
- Innate Immunity: Researchers commonly examine how CXCL16 (C-X-C motif chemokine 16) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CXCL16 (C-X-C motif chemokine 16) levels across conditions; band patterns may reflect isoforms and processing.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
