| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C-X-C motif chemokine 2;Cytokine-induced neutrophil chemoattractant 3;CINC-3;Macrophage inflammatory protein 2;MIP2;Cxcl2;Cinc3, Mip-2, Mip2, Scyb2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived rat CXCL2 recombinant protein (Position: S32-N100). Rat CXCL2 shares 62.3% and 88.4% amino acid (aa) sequence identity with huamn and mouse CXCL2, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Cxcl2 (C-X-C motif chemokine 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CXCL2 Antibody Picoband® catalog # PB9858. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived rat CXCL2 recombinant protein (Position: S32-N100). Rat CXCL2 shares 62.3% and 88.4% amino acid (aa) sequence identity with huamn and mouse CXCL2, respectively. (reported region: S32-N100).
- Molecular weight context: reported MW: 11 kDa; calculated MW: 10783 MW
- Reactivity: Mouse,Rat
- Applications: ELISA, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
C-X-C motif chemokine 2; C-X-C motif chemokine 2. Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2). CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2. Functional note: Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation. Reported localization: Secreted. Expression/tissue context: At least expressed in the lung and trachea.
Research relevance and current trends
- Chemokines: Researchers commonly examine how Cxcl2 (C-X-C motif chemokine 2) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how Cxcl2 (C-X-C motif chemokine 2) relates to this theme using model systems and orthogonal readouts.
- Innate Immunity: Researchers commonly examine how Cxcl2 (C-X-C motif chemokine 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Cxcl2 (C-X-C motif chemokine 2) levels across conditions; band patterns may reflect isoforms and processing.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
