| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-X-C chemokine receptor type 3; CXC-R3; CXCR-3; Interferon-inducible protein 10 receptor; IP-10 receptor; CD antigen CD183; Cxcr3; Cmkar3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Cxcr3 recombinant protein (Position: M1-E326). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects C-X-C chemokine receptor type 3 (gene Cxcr3) in mouse and rat samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 41.0 kDa, and the supplier reports an observed band at about 46 kDa.
CXCR3 is a G protein-coupled chemokine receptor for CXCL9, CXCL10 and CXCL11. It is expressed on activated T cells, especially Th1 and CD8+ effector cells, and on NK cells, and guides them into inflamed tissues and tumors. Alternative isoforms also bind CXCL4. It is studied in Th1 immunity, autoimmunity, transplantation and tumor immunology.
| Target | C-X-C chemokine receptor type 3 (gene Cxcr3; UniProt O88410, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 41.0 kDa |
| Observed MW | 46 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat C6 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse intestinal lymph node tissue, rat lymph node tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse Cxcr3 fragment (Met1–Glu326), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places C-X-C chemokine receptor type 3 in the cell membrane. Tissue expression noted by UniProt (mouse): Expresses in lymphoid organs and Th1 cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hongyan Yu et al. (2025) Interior Decorative VOCs Elevate T Cell-Mediated Obstructive Lung Disease Risks via Osteogenesis-Driven Lymphoid-Biased Hematopoiesis. Advanced Science. 10.1002/advs.202512663
Xi Yin et al. (2026) Docosahexaenoic Acid Attenuates Visceral Pain by Suppressing Spinal CXCL10/CXCR3/ERK Signaling. Nutrients. 10.3390/nu18071113
Mao‐mei Song et al. (2025) CXCL10 Involvement in Vestibular Migraine via the PI3K/AKT Signaling Pathway. CNS Neuroscience & Therapeutics. 10.1111/cns.70655
Kong Yan-Fang et al. (2020) CXCL10/CXCR3 Signaling in the DRG Exacerbates Neuropathic Pain in Mice. Neuroscience Bulletin. 10.1007/s12264-020-00608-1
Jing Peng-Bo et al. (2017) Chemokine Receptor CXCR3 in the Spinal Cord Contributes to Chronic Itch in Mice. Neuroscience Bulletin. 10.1007/s12264-017-0128-z
