| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; FB22; Fusin; HM89; LCR1; Leukocyte-derived seven transmembrane domain receptor; LESTR; Lipopolysaccharide-associated protein 3; LAP-3; LPS-associated protein 3; NPYRL; Stromal cell-derived factor 1 receptor; SDF-1 receptor; CD antigen CD184; CXCR4 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CXCR4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CID-3 is a rabbit monoclonal antibody that recognizes C-X-C chemokine receptor type 4 (gene CXCR4) in human and mouse samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 39.7 kDa, and the supplier reports an observed band at about 43 kDa.
CXCR4 is a G protein-coupled receptor for the chemokine CXCL12 (SDF-1). It controls retention and homing of hematopoietic stem cells in the bone marrow and guides lymphocyte trafficking and embryonic cell migration. CXCR4 is also a coreceptor for T-tropic HIV-1 and is widely studied in cancer metastasis and stem cell mobilization.
| Target | C-X-C chemokine receptor type 4 (gene CXCR4; UniProt P61073, human) |
|---|---|
| Clone | CID-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 39.7 kDa |
| Observed MW | 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:100–1:200 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): human cervical carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human CXCR4.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places C-X-C chemokine receptor type 4 in the cell membrane, cell junction, early endosome and late endosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Dongcai Wu et al. (2019) Hypoxia-induced microRNA-141 regulates trophoblast apoptosis, invasion, and vascularization by blocking CXCL12β/CXCR2/4 signal transduction. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2019.108836
