| Field | Specification |
|---|---|
| Target | |
| Alternative names | Atypical chemokine receptor 3;C-X-C chemokine receptor type 7;CXC-R7;CXCR-7;Chemokine orphan receptor 1;G-protein coupled receptor 159;G-protein coupled receptor RDC1 homolog;RDC-1;ACKR3;CMKOR1, CXCR7, GPR159, RDC1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CXCR7 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FEI-1 is a rabbit monoclonal antibody that recognizes atypical chemokine receptor 3 (gene ACKR3) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 41.5 kDa, and the supplier reports an observed band at about 38 kDa.
Atypical chemokine receptor 3 (ACKR3, CXCR7) binds CXCL12 and CXCL11 but signals mainly through beta-arrestin rather than G proteins. By scavenging CXCL12 it shapes gradients that guide migrating cells, and it is studied in development, cardiovascular biology and cancer.
| Target | Atypical chemokine receptor 3 (gene ACKR3; UniProt P25106, human) |
|---|---|
| Clone | FEI-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 41.5 kDa |
| Observed MW | 38 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human RT4 cells, human HeLa cells, human A431 cells, human MCF-7 cells, rat C6 cells, rat PC-12 cells, mouse NIH/3T3 cells, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human CXCR7.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places atypical chemokine receptor 3 in the cell membrane, early endosome and recycling endosome. Tissue expression noted by UniProt (human): Expressed in monocytes, basophils, B-cells, umbilical vein endothelial cells (HUVEC) and B-lymphoblastoid cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
