| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytochrome b-245 heavy chain;1.-.-.-;CGD91-phox;Cytochrome b (558) subunit beta;Cytochrome b558 subunit beta;Heme-binding membrane glycoprotein gp91phox;NADPH oxidase 2;Neutrophil cytochrome b 91 kDa polypeptide;Superoxide-generating NADPH oxidase heavy chain subunit;gp91-1;gp91-phox;p22 phagocyte B-cytochrome;CYBB;NOX2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CYBB |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GOH-3 is a rabbit monoclonal antibody that recognizes NADPH oxidase 2 (gene CYBB) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 65.3 kDa, and the supplier reports an observed band at about 65 kDa.
NOX2 (CYBB, gp91phox) is the catalytic, membrane-bound core of the phagocyte NADPH oxidase. When cytosolic partners assemble with it, it transfers electrons from NADPH to oxygen to generate superoxide, which helps neutrophils and macrophages kill microbes. Loss of CYBB causes X-linked chronic granulomatous disease, and NOX2 is studied in host defense and oxidative signaling.
| Target | NADPH oxidase 2 (gene CYBB; UniProt P04839, human) |
|---|---|
| Clone | GOH-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 65.3 kDa |
| Observed MW | 65 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human MCF-7 cells, human HeLa cells, human Jurkat cells, human HepG2 cells, rat liver tissue, rat brain tissue, mouse liver tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human CYBB.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places NADPH oxidase 2 in the cell membrane. Tissue expression noted by UniProt (human): Detected in neutrophils (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yadi Huang et al. (2026) Age- and diet-dependent progression of retinal microvascular injury in GCK-MODY under metabolic stress. Frontiers in Endocrinology. 10.3389/fendo.2026.1744691
Hua Zhou et al. (2020) Potential Protective Effects of the Water-Soluble Chinese Propolis on Hypertension Induced by High-Salt Intake. CTS-Clinical and Translational Science. 10.1111/cts.12770
Wansu Yu et al. (2024) In utero chronic intermittent nicotine aerosol exposure increases ischemic heart injury in adult offspring via programming of Angiotensin II receptor-derived TGFβ/ROS/Akt signaling pathway. Reproductive Toxicology. 10.1016/j.reprotox.2024.108650
