| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cytochrome b-245 heavy chain;1.-.-.-;CGD91-phox;Cytochrome b (558) subunit beta;Cytochrome b558 subunit beta;Heme-binding membrane glycoprotein gp91phox;NADPH oxidase 2;Neutrophil cytochrome b 91 kDa polypeptide;Superoxide-generating NADPH oxidase heavy chain subunit;gp91-1;gp91-phox;p22 phagocyte B-cytochrome;CYBB;NOX2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human CYBB |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CYBB/Nox2 Rabbit Monoclonal Antibody is an antibody targeting CYBB. Common applications include WB. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: GOH-3; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 47 kDa; calculated MW: 65336 MW.
Boster Bio Anti-CYBB/Nox2 Rabbit Monoclonal Antibody catalog # M00328. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: CYBB — Cytochrome b-245 heavy chain
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: GOH-3; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 47 kDa; Calculated: 65336 MW
Biological background
Protein function (datasheet): Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H (+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Cellular localization (datasheet): Cell membrane; Multi-pass membrane protein.
Tissue details (datasheet): Detected in neutrophils (at protein level). .
Research relevance and current trends
- Commonly studied in contexts related to Adaptive Immunity,Cancer,Cancer Metabolism,Immunology,Metabolism,T Cells.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
