| Field | Specification |
|---|---|
| Target | |
| Alternative names | G1/S-specific cyclin-D3;CCND3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Cyclin D3 recombinant protein (Position: Q136-L292). Human Cyclin D3 shares 94% and 93% amino acid (aa) sequences identity with mouse and rat Cyclin D3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets G1/S-specific cyclin-D3 (gene CCND3) in human and rat samples and is validated for Western blot. The predicted molecular weight is 32.5 kDa, and the supplier reports an observed band at about 33 kDa.
Cyclin D3 (CCND3) is a D-type cyclin that forms complexes with CDK4 or CDK6 to phosphorylate RB family proteins and drive the G1/S transition. It is especially prominent in lymphocytes and hematopoietic cells, where it supports proliferation during development and immune responses.
| Target | G1/S-specific cyclin-D3 (gene CCND3; UniProt P30281, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 32.5 kDa |
| Observed MW | 33 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): rat testis tissue, rat thymus tissue, rat lung tissue, rat ovary tissue, Jurkat cells, A549 cells, MCF-7 cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human Cyclin D3 fragment (Gln136–Leu292), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 94% and 93%, respectively.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places G1/S-specific cyclin-D3 in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Haolong Zhang et al. (2018) Wall shear stress promotes intimal hyperplasia through the paracrine H2O2-mediated NOX-AKT-SVV axis. Life Sciences. 10.1016/j.lfs.2018.05.045
Luo Yanxia et al. (2018) Co-culture with TM4 cells enhances the proliferation and migration of rat adipose-derived mesenchymal stem cells with high stemness. Cytotechnology. 10.1007/s10616-018-0235-3
