| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin-dependent kinase 6;2.7.11.22;CR2 protein kinase;CRK2;Cell division protein kinase 6;Serine/threonine-protein kinase PLSTIRE;Cdk6;Cdkn6, Crk2; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cdk6, identical to the related rat sequence, and different from the related human sequence by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
This rabbit polyclonal antibody detects cyclin-dependent kinase 6 (gene Cdk6) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 37.0 kDa, and the supplier reports an observed band at about 100 kDa.
CDK6 is a serine/threonine kinase that forms complexes with D-type cyclins to phosphorylate RB1 and move cells from G1 into S phase. Besides its cell cycle role it influences differentiation, particularly in blood cell development, and it is a key target in cancer research alongside CDK4.
| Target | Cyclin-dependent kinase 6 (gene Cdk6; UniProt Q64261, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 37.0 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat, Mouse) |
|---|
Samples with a confirmed band (WB): rat testis tissue, rat lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the C-terminal region of mouse cdk6. The peptide sequence is conserved in rat. It differs from the human sequence at four residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cyclin-dependent kinase 6 in the cytoplasm, nucleus and cell projection. Tissue expression noted by UniProt (mouse): Expressed in subgranular zone (SGZ) of the hippocampal dentate gyrus (DG) and the subventricular zone (SVZ) of the lateral ventricles whose neural precursor cells (NPC) give rise to dentate granule neurons and olfactory bulb (OB) interneurons, respectively. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yueling Tang et al. (2023) Role and mechanism of Circ-PDE7B in the formation of keloid. International Wound Journal. 10.1111/iwj.14269
