| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Peptidyl-prolyl cis-trans isomerase B;PPIase B;5.2.1.8;CYP-S1;Cyclophilin B;Rotamase B;S-cyclophilin;SCYLP;PPIB;CYPB; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Cyclophilin B |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-PPIB antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone DOO-16; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB (as provided in the source record). Boster Bio Anti-Cyclophilin B PPIB Rabbit Monoclonal Antibody catalog # M03229. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: PPIB (Peptidyl-prolyl cis-trans isomerase B).
- Antibody format: Monoclonal; clone DOO-16; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
PPIB (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Reported cellular localization context: Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue expression notes (as provided): Expressed in brain in the thalamus, in fetal and adult lung and in placenta. .
Research relevance and current trends
- Research context keywords from the source record include: ER Proteins,Immunology,Protein Trafficking,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
Workflow ideas (metafield): Validate PPIB antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PPIB expression by Western blot in cell or tissue lysates, Compare relative PPIB levels across experimental conditions (dose/time-course) using antibody-based readouts
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 100 kDa; calculated MW: 23743 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 100 kDa
- Cellular localization (provided): Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Tissue details (provided): Expressed in brain in the thalamus, in fetal and adult lung and in placenta. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
