| Field | Specification |
|---|---|
| Target | |
| Alternative names | Peptidyl-prolyl cis-trans isomerase B;PPIase B;5.2.1.8;CYP-S1;Cyclophilin B;Rotamase B;S-cyclophilin;SCYLP;PPIB;CYPB; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cyclophilin B |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DOO-16 is a rabbit monoclonal antibody that recognizes peptidyl-prolyl cis-trans isomerase B (gene PPIB) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 23.7 kDa, and the supplier reports an observed band at about 18 kDa.
Cyclophilin B (PPIB) is a peptidyl-prolyl cis-trans isomerase that resides in the endoplasmic reticulum. It speeds up folding of secretory proteins, notably collagen, by isomerizing proline bonds. PPIB mutations cause a recessive form of osteogenesis imperfecta, and it is studied in protein folding and collagen biology.
| Target | Peptidyl-prolyl cis-trans isomerase B (gene PPIB; UniProt P23284, human) |
|---|---|
| Clone | DOO-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 23.7 kDa |
| Observed MW | 18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human cyclophilin B.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places peptidyl-prolyl cis-trans isomerase B in the virion. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
