| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cystathionine gamma-lyase;4.4.1.1;Cysteine-protein sulfhydrase;Gamma-cystathionase;CTH; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Cystathionase recombinant protein (Position: D181-H398). Human Cystathionase shares 85.8% amino acid (aa) sequence identity with both mouse and rat Cystathionase. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CTH (Cystathionine gamma-lyase) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Cystathionase/CTH Antibody Picoband® catalog # PB9494. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human Cystathionase recombinant protein (Position: D181-H398). Human Cystathionase shares 85.8% amino acid (aa) sequence identity with both mouse and rat Cystathionase. (reported region: D181-H398).
- Molecular weight context: reported MW: 45 kDa; calculated MW: 44508 MW
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Cystathionine gamma-lyase; Cystathionine gamma-lyase. Cystathionine gamma-lyase (or cystathionase) is an enzyme which breaks down cystathionine into cysteine and α-ketobutyrate. The International Radiation Hybrid Mapping Consortium mapped the CTH gene to chromosome 1. The CTH gene had earlier been assigned to chromosome 16 by study of somatic cell hybrids. It is demonstrated that hydrogen sulfide (H2S) is physiologically generated by CTH. Functional note: Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function. . Reported localization: Cytoplasm. Expression/tissue context: Ubiquitous. Absent in primary spermatocytes. .
Research relevance and current trends
- Cancer: Researchers commonly examine how CTH (Cystathionine gamma-lyase) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how CTH (Cystathionine gamma-lyase) relates to this theme using model systems and orthogonal readouts.
- Collagen: Researchers commonly examine how CTH (Cystathionine gamma-lyase) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CTH (Cystathionine gamma-lyase) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the CTCF zinc-finger protein family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
