| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cystatin-A;Cystatin-AS;Stefin-A;Cystatin-A, N-terminally processed;CSTA;STF1, STFA; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Cystatin A recombinant protein (Position: E8-F98). Human Cystatin A shares 61.5% and 58.2% amino acid (aa) sequence identity with mouse and rat Cystatin A, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CSTA (Cystatin-A) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Cystatin A/CSTA Antibody Picoband® catalog # PB9564. Tested in ICC/IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human Cystatin A recombinant protein (Position: E8-F98). Human Cystatin A shares 61.5% and 58.2% amino acid (aa) sequence identity with mouse and rat Cystatin A, respectively. (reported region: E8-F98).
- Molecular weight context: reported MW: 11 kDa; calculated MW: 11 kDa
- Reactivity: Human,Mouse,Rat
- Applications: ICC/IF, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Cystatin-A; Cystatin-A. Cystatin A also called Stefin A is a protein that in humans is encoded by the CSTA gene. Cystatin A is a cysteine proteinase inhibitor that belongs to family 1 of the cystatin superpamily. This gene was assigned to human chromosome 3q21.1. This is an intracellular thiol proteinase inhibitor. Cystatin A plays an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis. Stefins have been proposed as prognostic and diagnostic tools for cancer. Functional note: This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis. . Reported localization: Cytoplasm . Expression/tissue context: Expressed in the skin throughout the epidermis. .
Research relevance and current trends
- Apoptosis: Researchers commonly examine how CSTA (Cystatin-A) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how CSTA (Cystatin-A) relates to this theme using model systems and orthogonal readouts.
- Caspases: Researchers commonly examine how CSTA (Cystatin-A) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CSTA (Cystatin-A) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the cystatin family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
