| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytochrome P450 1A2; CYPIA2; Cholesterol 25-hydroxylase; Cytochrome P(3)450; Cytochrome P450 4; Cytochrome P450-P3; Hydroperoxy icosatetraenoate dehydratase; CYP1A2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cytochrome P450 1A2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFAI-3 is a rabbit monoclonal antibody that recognizes cytochrome P450 1A2 (gene CYP1A2) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 58.4 kDa, and the supplier reports an observed band at about 52 kDa.
CYP1A2 is a liver cytochrome P450 enzyme that metabolizes many drugs and chemicals, including caffeine, theophylline and some aromatic amines, and it also acts on estrogens and fatty acids. Its expression is induced through the aryl hydrocarbon receptor, for example by smoking, so it is studied in drug metabolism, drug interactions and toxicology.
| Target | Cytochrome P450 1A2 (gene CYP1A2; UniProt P05177, human) |
|---|---|
| Clone | AFAI-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 58.4 kDa |
| Observed MW | 52 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:500 |
Immunogen
Synthetic peptide based on the sequence of human cytochrome P450 1A2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places cytochrome P450 1A2 in the endoplasmic reticulum membrane and microsome membrane. Tissue expression noted by UniProt (human): Liver.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
