| Field | Specification |
|---|---|
| Target | |
| Alternative names | NADPH--cytochrome P450 reductase; CPR; P450R; POR; CYPOR |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cytochrome P450 Reductase. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEFH-16 is a rabbit monoclonal antibody that recognizes NADPH--cytochrome P450 reductase (gene POR) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 76.7 kDa, and the supplier reports an observed band at about 77 kDa.
Cytochrome P450 oxidoreductase (POR) is an ER membrane flavoprotein that transfers electrons from NADPH to microsomal cytochrome P450 enzymes. It also supplies electrons to heme oxygenase and cytochrome b5. POR is therefore required for drug metabolism and steroid synthesis, and its mutations cause a disorder of steroid production.
| Target | NADPH--cytochrome P450 reductase (gene POR; UniProt P16435, human) |
|---|---|
| Clone | AEFH-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 76.7 kDa |
| Observed MW | 77 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human cytochrome P450 Reductase.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places NADPH--cytochrome P450 reductase in the endoplasmic reticulum membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
