| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; K8; Type-II keratin Kb8; KRT8; CYK8 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Cytokeratin 8 recombinant protein (Position: D107-K325). Human Cytokeratin 8 shares 95.4% and 94.5% amino acid (aa) sequence identity with mouse and rat Cytokeratin 8, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of KRT8 (CD79b molecule) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Cytokeratin 8 KRT8 Antibody Picoband® (monoclonal, 3G9) catalog # M01421-3. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Mouse Monoclonal Mouse IgG2b
- Clone number: Clone: 3G9
- Immunogen / epitope context: E.coli-derived human Cytokeratin 8 recombinant protein (Position: D107-K325). Human Cytokeratin 8 shares 95.4% and 94.5% amino acid (aa) sequence identity with mouse and rat Cytokeratin 8, respectively. (reported region: D107-K325).
- Molecular weight context: reported MW: 54 kDa; calculated MW: nan
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IF, IHC, IHC-F, ICC, WB
As a monoclonal antibody, the reagent targets a defined epitope, supporting consistency across experiments; epitope masking by PTMs or conformational changes can affect signal.
Biological background
CD79b molecule; keratin 8. Keratin, type II cytoskeletal 8, also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that is encoded in humans by the KRT8 gene. This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. Functional note: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Reported localization: Nucleoplasm. Nucleus matrix. Cytoplasm. Expression/tissue context: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Research relevance and current trends
- Class I: Researchers commonly examine how KRT8 (CD79b molecule) relates to this theme using model systems and orthogonal readouts.
- Cytoskeleton: Researchers commonly examine how KRT8 (CD79b molecule) relates to this theme using model systems and orthogonal readouts.
- Cytoskeleton/ECM: Researchers commonly examine how KRT8 (CD79b molecule) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative KRT8 (CD79b molecule) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of KRT8 (CD79b molecule) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
