| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Keratin, type II cytoskeletal 7;Cytokeratin-7;CK-7;Keratin-7;K7;Type-II keratin Kb7;Krt7 ;Kb7 , Krt2-7 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Cytoskeletal preparation of the RT4 human bladder carcinoma cell line. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Cytokeratin Peptide 7 KRT7 Antibody (Monoclonal, LDS-68) is an antibody targeting KRT7. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: LDS-68; isotype: Mouse IgG1; reactivity: Human; observed MW: 37 kDa; calculated MW: 50709 MW.
Boster Bio Anti-Cytokeratin Peptide 7 KRT7 Antibody (Monoclonal, LDS-68) catalog # MA1024. Tested in IHC, WB applications. This antibody reacts with Human.
Key elements and design rationale
- Target: KRT7 — Keratin, type II cytoskeletal 7
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: LDS-68; Isotype: Mouse IgG1
- Species reactivity: Human
- Molecular weight guidance: Observed: 37 kDa; Calculated: 50709 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. .
Scientific background (datasheet): KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans.
Cellular localization (datasheet): KRT7: Cytoplasm
Tissue details (datasheet): KRT7: Expressed in cultured epidermal, bronchial andmesothelial cells but absent in colon, ectocervix and liver.Observed throughout the glandular cells in the junction betweenstomach and esophagus but is absent in the esophagus.
Sequence similarities (datasheet): Belongs to the intermediate filament family.
Research relevance and current trends
- Commonly studied in contexts related to Class I,Cytoskeleton,Cytoskeleton/ECM,Intermediate Filaments,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
