| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein DBF4 homolog A; Activator of S phase kinase; Chiffon homolog A; DBF4-type zinc finger-containing protein 1; DBF4; ASK; DBF4A; ZDBF1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DBF4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24D74 is a rabbit monoclonal antibody that recognizes protein DBF4 homolog A in human samples and is validated for Western blot. The predicted molecular weight is 76.9 kDa, and the supplier reports an observed band at about 77 kDa.
DBF4 is the regulatory subunit that switches on the kinase CDC7, forming the DBF4-dependent kinase (DDK). This complex phosphorylates the MCM2-7 helicase at replication origins, a step required to fire origins and start DNA synthesis in S phase. DBF4 is therefore studied in DNA replication control, cell proliferation and as part of a kinase targeted in cancer research.
| Target | Protein DBF4 homolog A (gene DBF4; UniProt Q9UBU7, human) |
|---|---|
| Clone | 24D74 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 76.9 kDa |
| Observed MW | 77 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DBF4.
Reactivity Notes
The supplier lists reactivity with human. UniProt places protein DBF4 homolog A in the nucleus. Tissue expression noted by UniProt (human): Highly expressed in testis and thymus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
