| Field | Specification |
|---|---|
| Target | |
| Alternative names | Proto-oncogene DBL; Proto-oncogene MCF-2; MCF2; DBL |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human DBL recombinant protein (Position: D42-Y925). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets proto-oncogene DBL (gene MCF2) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 107.7 kDa, and the supplier reports an observed band at about 125 kDa.
MCF2, also known as DBL, is a guanine nucleotide exchange factor that activates Rho family GTPases such as RhoA and CDC42 by promoting GDP-to-GTP exchange. It gives its name to the DH (Dbl homology) domain found in many Rho activators. The protein was originally discovered as a transforming oncogene, and it is studied in cytoskeletal signaling and cell transformation.
| Target | Proto-oncogene DBL (gene MCF2; UniProt P10911, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 107.7 kDa |
| Observed MW | 125 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): MCF-7 cells, U251 cells.
Immunogen
Recombinant human DBL fragment (Asp42–Tyr925), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places proto-oncogene DBL in the cytoplasm and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (human): Isoform 1 is expressed only in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
