| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial; 2.3.1.168; 52 kDa mitochondrial autoantigen of primary biliary cirrhosis; Branched chain 2-oxo-acid dehydrogenase complex component E2; BCOADC-E2; Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; BCKDH-E2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase; DBT; BCATE2; BCKDHE2 |
| Clonality | |
| Form | Liquid |
| Host | |
| Immunogen | E. coli - derived recombinant human DBT (Met1-Lys257). |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Overview
Anti-DBT Polyclonal Antibody targets DBT (Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex). Frequently used across ELISA, IHC, WB workflows, depending on experimental design and sample type. This antibody is suited for workflows commonly used in Molecular & Cellular Biology research.
Also known as: Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, 52 kDa mitochondrial autoantigen of primary biliary cirrhosis, Branched-chain alpha-keto acid dehydrogenase complex component E2, Branched chain 2-oxo-acid dehydrogenase complex component E2, Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase, Dihydrolipoamide branched chain transacylase, BCOADC-E2, BCKAD-E2, BCKDH-E2.
Target biology
DBT (Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex) is a modification-specific target used to assess protein regulatory state rather than total protein abundance. Post-translational modification epitopes can be stimulation- and fixation-sensitive; rapid sample processing and appropriate inhibitors can help preserve signal. Common alternative names and symbols used in the literature include: Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, 52 kDa mitochondrial autoantigen of primary biliary cirrhosis, Branched-chain alpha-keto acid dehydrogenase complex component E2, Branched chain 2-oxo-acid dehydrogenase complex component E2, Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase, Dihydrolipoamide branched chain transacylase, BCOADC-E2, BCKAD-E2, BCKDH-E2, mitochondrial. Based on the annotated name/class, the target is most often associated with mitochondria localization, though this can vary by model system.
How this antibody helps
As a polyclonal reagent, this antibody can provide strong sensitivity through recognition of multiple epitopes on the target. For best performance, interpret signal in the context of appropriate biological controls and orthogonal readouts when available.
Antigen / immunogen context
E. coli - derived recombinant human DBT (Met1-Lys257).
Recommended experimental notes
- Titrate the antibody to optimize signal-to-noise for your specific sample type and detection system.
- Use appropriate positive and negative controls (e.g., target-high vs target-low samples, KO/KD where available).
- For Western blotting, use fresh protease (and phosphatase, if relevant) inhibitors and confirm the primary band at the expected molecular weight; additional bands may reflect isoforms or processing.
- For IHC, optimize fixation and antigen retrieval (buffer/pH/heat) and include an isotype/secondary-only control to assess background.
- For ELISA, optimize capture/detection pairing and run a standard curve in matrix-matched diluent when possible.
Additional notes
For research use only
For research use only. Not for diagnostic procedures.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
