Anti-DDX3 antibody (100-200 aa) [SRM5180] (STJ11107047)

What it means: Monoclonal antibodies originate from a single hybridoma clone that recognizes one defined epitope on the target antigen — making Monoclonal antibodies uniquely consistent between lots.

Why it matters:

• Monoclonal antibodies offer lot-to-lot reproducibility that is critical for longitudinal studies, quantitative assays, and any experimental design requiring matched performance across timepoints or collaborating labs.
• The defined single-epitope binding profile enables reliable pairing with a non-competing capture antibody for sandwich ELISA, proximity ligation, and flow cytometry multiplex panels.

How to interpret here: A single, well-optimized titration for this Monoclonal antibody transfers reliably across experiments. Confirm whether the target epitope is preserved under your fixation or denaturation conditions before proceeding with IHC or WB applications.

What it means: This antibody was raised in Rabbit, producing high-affinity IgG with long CDR3 loops that confer exceptional epitope recognition. Anti-rabbit HRP, fluorochrome, and biotin secondary antibodies are universally available across all major suppliers.

Why it matters:

• The Rabbit host species determines which secondary antibody you need for indirect detection — select an anti-Rabbit IgG secondary matched to your detection label (HRP, fluorochrome, or biotin).
• Rabbit-derived antibodies pair optimally with anti-rabbit secondary antibodies. Avoid using rabbit-derived secondary antibodies in experiments involving rabbit tissue — use species-absorbed secondaries or recombinant secondaries instead.

How to interpret here: Confirm availability of an anti-Rabbit secondary antibody in your lab before ordering. For multiplex IF, pair this Rabbit-raised antibody with antibodies from a different host species to avoid secondary antibody cross-reactivity.

What it means: Validated reactivity to Human/Mouse/Rat — this antibody cross-reacts with orthologs from multiple species within a single reagent.

Why it matters:

• Cross-species reactivity (Human/Mouse/Rat) allows direct comparison of the same target across Human, Mouse and Rat experimental models without switching antibodies, eliminating an important source of inter-study variability.
• All listed reactivities are experimentally confirmed, not computationally predicted — critical for reviewers and reproducibility standards.

How to interpret here: Confirm that your specific species is among the validated list (Human/Mouse/Rat). Reactivity to unlisted species should be independently validated before use in publication-quality data.

What it means: Unconjugated format means no detection label is attached to this antibody — you supply a compatible secondary antibody or detection reagent of your choice.

Why it matters:

• The Unconjugated format gives maximum flexibility: pair with HRP-, fluorochrome-, or biotin-conjugated secondary antibodies matched to your detection platform, enabling use across WB, IHC, IF, and ELISA without ordering separate conjugated primary formats.
• Signal amplification is possible by using a biotinylated secondary followed by a streptavidin-enzyme conjugate, which is not available with directly conjugated primary antibodies.

How to interpret here: Select an anti-host-species secondary antibody conjugated to your preferred detection label. For most indirect detection workflows, the Unconjugated primary antibody is the recommended starting format.

What it means: IgG is the immunoglobulin class and subclass of this antibody, defining its constant-region structure and effector functions.

Why it matters:

• Isotype IgG determines which secondary antibody constant-region specificities are compatible — isotype-specific anti-IgG1 or anti-IgG2a secondaries enable discrimination of co-stained antibodies from the same host species in multiplex experiments.
• For functional assays, isotype identity guides selection of Fc-receptor binding controls and isotype-matched negative controls required for rigorous experimental design.

How to interpret here: Use a negative control of the same isotype (IgG) to assess non-specific binding in your assay. If performing multiplex staining with multiple antibodies from the same species, select isotype-specific secondary antibodies to resolve each IgG signal independently.

What it means: Affinity purification means this antibody was isolated using antigen- or protein-specific ligand chromatography, selectively retaining immunoglobulins that bind the target.

Why it matters:

• Affinity purification removes non-specific serum proteins and irrelevant immunoglobulins, delivering a high-purity preparation that minimizes background noise in sensitive applications such as IHC, IF, and flow cytometry.
• Antibodies purified by Affinity purification methods generally exhibit higher specific activity per milligram, allowing use at lower concentrations — important for reducing cost-per-experiment in large-scale studies.

How to interpret here: The Affinity purification process confirms that the active immunoglobulin fraction has been enriched. Follow the recommended dilution on the datasheet; avoid over-concentrating in reconstitution to maintain solubility.

What it means: SRM5180 is the unique clone designation of this monoclonal antibody, identifying the specific hybridoma lineage that defines its epitope and performance characteristics.

Why it matters:

• Clone SRM5180 allows precise cross-referencing with published literature — if peer-reviewed studies cite this specific clone, its performance in your assay context is already independently validated.
• Recording clone SRM5180 in your methods section enables collaborators and reviewers to replicate your exact reagent, satisfying increasingly stringent journal antibody reporting requirements (RRID registration, clone number disclosure).

How to interpret here: Search clone SRM5180 in published literature and antibody validation databases to review independent performance data. Always record the full clone designation in experimental notes for complete reproducibility documentation.