| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Peptidyl-prolyl cis-trans isomerase D; PPIase D |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human DDX4/MVH recombinant protein (Position: D3-D666). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-DDX4/MVH Antibody Picoband® (monoclonal, 3C3) is an antibody for DDX4 detection raised in Mouse (Monoclonal, clone Clone: 3C3, Mouse IgG2b), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, IF, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: DDX4 (peptidylprolyl isomerase D); UniProt: Q9NQI0
- Antibody format: Mouse, Monoclonal, clone Clone: 3C3, Mouse IgG2b
- Molecular weight: 79 kDa
- Applications: WB, IHC, IF, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-DDX4/MVH Antibody Picoband® (monoclonal, 3C3) catalog # M02448-1.
Biological background
Biological context: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Proposed to act as a co-chaperone in HSP90 complexes such as in unligated steroid receptors heterocomplexes. Different co-chaperones seem to compete for association with HSP90 thus establishing distinct HSP90-co-chaperone-receptor complexes with the potential to exert tissue-specific receptor activity control. May have a preference for estrogen receptor complexes and is not found in glucocorticoid receptor complexes. May be involved in cytoplasmic dynein-dependent movement of the receptor from the cytoplasm to the nucleus. May regulate MYB by inhibiting its DNA- binding activity. Involved in regulation of AHR signaling by promoting the formation of the AHR:ARNT dimer; the function is independent of HSP90 but requires the chaperone activity. Involved in regulation of UV radiation-induced apoptosis. Promotes cell viability in anaplastic lymphoma kinase-positive anaplastic large- cell lymphoma (ALK+ ALCL) cell lines. May be involved in hepatitis C virus (HCV) replication and release.
Expression and localization notes: cellular localization: Cytoplasm, tissue context: Widely expressed..
Common research applications
- Western blotting (WB): Compare DDX4 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of DDX4 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify DDX4-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: DDX4(DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al.(2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog(Ddx4) resides(Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoplasm
- Tissue details: Widely expressed.
- Research category: Immunology
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
