| Field | Specification |
|---|---|
| Target | |
| Alternative names | Histone H2AX;H2a/x;Histone H2A.X;H2AFX;H2AX; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DDX58 Retinoic acid inducible gene I (RIG-I) is a 925 amino acid, interferon-inducible cellular DExD/H box RNA helicase that activates type I interferon (IFN), an important effector of the innate immune system that is sensitive to these dsRNA viruses. dsRNA is normally present in very low quantities in cells, so when a virus is present, the elevated levels of dsRNA act as a sign telling RIG-I to activate the production of IFN. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACEA-4 is a rabbit monoclonal antibody that recognizes antiviral innate immune response receptor RIG-I (gene RIGI) in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 106.6 kDa, and the supplier reports an observed band at about 107 kDa.
RIG-I (RIGI, previously DDX58) is a cytosolic RNA helicase that detects viral RNA, particularly short double-stranded RNA with a 5′-triphosphate end. On binding, it oligomerizes and engages the adaptor MAVS on mitochondria through its CARD domains, triggering IRF3 and NF-κB activation and production of type I interferons. It is a key sensor in antiviral innate immunity research.
| Target | Antiviral innate immune response receptor RIG-I (gene RIGI; UniProt O95786, human) |
|---|---|
| Clone | ACEA-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 106.6 kDa |
| Observed MW | 107 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human Jurkat cells, human SIHA cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human DDX58.
Reactivity Notes
The supplier lists reactivity with human. UniProt places antiviral innate immune response receptor RIG-I in the cytoplasm, cell projection and cell junction. Tissue expression noted by UniProt (human): Present in vascular smooth cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
