| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Desmin;Des; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Desmin from pig stomach. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Desmin Antibody (Monoclonal, DE-U-10) is an antibody targeting DES. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: DE-U-10; isotype: Mouse IgG1; reactivity: Human,Mouse,Rabbit,Rat; observed MW: 53 kDa; calculated MW: 53457 MW.
Boster Bio Anti-Desmin Antibody (Monoclonal, DE-U-10) catalog # MA1036. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.
Key elements and design rationale
- Target: DES — Desmin
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: DE-U-10; Isotype: Mouse IgG1
- Species reactivity: Human,Mouse,Rabbit,Rat
- Molecular weight guidance: Observed: 53 kDa; Calculated: 53457 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. .
Scientific background (datasheet): Desmin belongs to the type III family of intermediate filaments, a class of cytoskeletal elements. DES gene encodes desmin, a muscle-specific cytoskeletal protein found in smooth, cardiac, and heart muscles. Tidball (1992) found that desmin was codistributed with actin thin filaments within the cellular processes of myotendinous junctions in frog skeletal muscle. DES gene contains 9 exons and spans about 8.4 kb. By in situ hybridization, Viegas-Pequignot et al. (1989) localized the gene to 2q35. Desmin mutation responsible for idiopathic dilated cardiomyopathy.
Cellular localization (datasheet): Cytoplasm, myofibril, sarcomere, Z line . Cytoplasm . Cell membrane, sarcolemma . Localizes in the intercalated disks which occur at the Z line of cardiomyocytes. .
Sequence similarities (datasheet): Belongs to the intermediate filament family.
Research relevance and current trends
- Commonly studied in contexts related to Complement,Immunology,Innate Immunity.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
