| Field | Specification |
|---|---|
| Target | |
| Alternative names | Desmoglein-2; Cadherin family member 5; HDGC; DSG2; CDHF5 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Desmoglein 2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFBF-4 is a rabbit monoclonal antibody that recognizes desmoglein-2 (gene DSG2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 122.3 kDa, and the supplier reports an observed band at about 160 kDa.
Desmoglein-2 (DSG2) is the most widely expressed desmosomal cadherin, present in simple epithelia and heart muscle. It links desmosomal plaque proteins to intermediate filaments to support cell-cell adhesion, and it is studied in epithelial biology and arrhythmogenic cardiomyopathy.
| Target | Desmoglein-2 (gene DSG2; UniProt Q14126, human) |
|---|---|
| Clone | AFBF-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 122.3 kDa |
| Observed MW | 160 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human desmoglein 2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places desmoglein-2 in the cell membrane, cell junction and cytoplasm. Tissue expression noted by UniProt (human): Expressed in undifferentiated pluripotent stem cells, expression decreases during differentiation (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
