| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Desmoplakin; DP; 250/210 kDa paraneoplastic pemphigus antigen; DSP |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human Desmoplakin recombinant protein (Position: Q1810-A2092). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of DSP (Steroid 17-alpha-hydroxylase/17,20 lyase) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Desmoplakin/DSP Antibody Picoband® catalog # A00616-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human Desmoplakin recombinant protein (Position: Q1810-A2092). (reported region: Q1810-A2092).
- Molecular weight context: reported MW: 250-280 kDa; calculated MW: 57371 MW
- Reactivity: Human,Mouse,Rat
- Applications: ELISA, IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Steroid 17-alpha-hydroxylase/17,20 lyase; desmoplakin. Desmoplakin is a protein in humans that is encoded by the DSP gene. This gene encodes a protein that anchors intermediate filaments to desmosomal plaques and forms an obligate component of functional desmosomes. Mutations in this gene are the cause of several cardiomyopathies and keratodermas, including skin fragility-woolly hair syndrome. Alternative splicing results in multiple transcript variants. Functional note: Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin- plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes. Reported localization: Cell junction, desmosome. Expression/tissue context: Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin.
Research relevance and current trends
- Calcium Binding Proteins: Researchers commonly examine how DSP (Steroid 17-alpha-hydroxylase/17,20 lyase) relates to this theme using model systems and orthogonal readouts.
- Calcium Signaling: Researchers commonly examine how DSP (Steroid 17-alpha-hydroxylase/17,20 lyase) relates to this theme using model systems and orthogonal readouts.
- Cell Cycle: Researchers commonly examine how DSP (Steroid 17-alpha-hydroxylase/17,20 lyase) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative DSP (Steroid 17-alpha-hydroxylase/17,20 lyase) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of DSP (Steroid 17-alpha-hydroxylase/17,20 lyase) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
